Strategies for analyzing bisulfite sequencing data
نویسندگان
چکیده
منابع مشابه
Strategies for analyzing bisulfite sequencing data.
DNA methylation is one of the main epigenetic modifications in the eukaryotic genome; it has been shown to play a role in cell-type specific regulation of gene expression, and therefore cell-type identity. Bisulfite sequencing is the gold-standard for measuring methylation over the genomes of interest. Here, we review several techniques used for the analysis of high-throughput bisulfite sequenc...
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dna methylation is an important biological process involving in human disease such as cancer insomia and diabetes. bisulfite sequencing (bs-seq) with next-generation technology is an accurate method for measuring dna methylation. bs-seq data analysis is a considerable way to recognize methylated cytosines and several tools have been developed to analysis bs-seq such as bs-seeker, b-solana, brat...
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Whole-Genome Bisulfite Sequencing (WGBS) and genome-wide Reduced Representation Bisulfite Sequencing (RRBS) are widely used to study DNA methylation. However, data analysis is complicated, lengthy, and hampered by a lack of seamless analytical pipelines. To address these issues, we developed a convenient, stable, and efficient web service called Web Service for Bisulfite Sequencing Data Analysi...
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The emerging genome-wide hairpin bisulfite sequencing (hairpin-BS-Seq) technique enables the determination of the methylation pattern for DNA double strands simultaneously. Compared with traditional bisulfite sequencing (BS-Seq) techniques, hairpin-BS-Seq can determine methylation fidelity and increase mapping efficiency. However, no computational tool has been designed for the analysis of hair...
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Next-generation sequencing technology has enabled genome-wide analysis of 5mC nucleotides at single-nucleotide resolution. Previous methods for whole-genome bisulfite sequencing (WGBS) yielded reduced genomic representation due to required DNA shearing, ligation of methylated sequencing adapters, and bisulfite conversion of unmethylated cytidine residues prior to sequencing. One disadvantage of...
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ژورنال
عنوان ژورنال: Journal of Biotechnology
سال: 2017
ISSN: 0168-1656
DOI: 10.1016/j.jbiotec.2017.08.007